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luciferase-based pglosensor-22f camp reporter plasmid glosensor  (Promega)

 
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    Promega luciferase-based pglosensor-22f camp reporter plasmid glosensor
    Luciferase Based Pglosensor 22f Camp Reporter Plasmid Glosensor, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luciferase-based pglosensor-22f camp reporter plasmid glosensor/product/Promega
    Average 90 stars, based on 1 article reviews
    luciferase-based pglosensor-22f camp reporter plasmid glosensor - by Bioz Stars, 2026-05
    90/100 stars

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    luciferase-based pglosensor-22f camp reporter plasmid glosensor  (Promega)
    90
    Promega luciferase-based pglosensor-22f camp reporter plasmid glosensor
    Luciferase Based Pglosensor 22f Camp Reporter Plasmid Glosensor, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luciferase-based pglosensor-22f camp reporter plasmid glosensor/product/Promega
    Average 90 stars, based on 1 article reviews
    luciferase-based pglosensor-22f camp reporter plasmid glosensor - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier
    luciferase-based glosensor camp reporter  (Promega)
    90
    Promega luciferase-based glosensor camp reporter
    Luciferase Based Glosensor Camp Reporter, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luciferase-based glosensor camp reporter/product/Promega
    Average 90 stars, based on 1 article reviews
    luciferase-based glosensor camp reporter - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier
    luciferase based camp reporter glosensor 22f  (Promega)
    90
    Promega luciferase based camp reporter glosensor 22f
    ( A ) Confocal images showing cell surface expression in mammalian HEK293 cells of 5-HT receptors cloned from S. mansoni ( Sm .5HTR L ), S. haematobium ( Sh. 5HTR) and S. japonicum ( Sj .5HTR) localized by COOH-terminally tagged eGFP. Scalebar, 50 µm. ( B ) Schematic of luminescent cAMP sensor bioassay. cAMP generated by schistosome 5-HTRs (blue) binds the engineered GloSensor <t>luciferase,</t> switching the sensor to a more active conformation resulting in enhanced luminescence output. ( C ) Kinetics of signal following the addition of 5-HT (1 µM) to HEK293 cells co-transfected with luminescent cAMP sensor and individual schistosome 5-HT receptors. Open circles, cells not transfected with 5-HT receptor, colored circles represent measurements in cells transfected with Sm .5HTR L (black), ( Sh. 5HTR (blue) and Sj .5HTR (red). ( D ) Serotonin dose-response curves for each of the three receptors (colored circles) and HEK293 cells expressing the cAMP sensor alone (open circles). Data reflect mean ± standard error of at least 3 biological replicates.
    Luciferase Based Camp Reporter Glosensor 22f, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luciferase based camp reporter glosensor 22f/product/Promega
    Average 90 stars, based on 1 article reviews
    luciferase based camp reporter glosensor 22f - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Confocal images showing cell surface expression in mammalian HEK293 cells of 5-HT receptors cloned from S. mansoni ( Sm .5HTR L ), S. haematobium ( Sh. 5HTR) and S. japonicum ( Sj .5HTR) localized by COOH-terminally tagged eGFP. Scalebar, 50 µm. ( B ) Schematic of luminescent cAMP sensor bioassay. cAMP generated by schistosome 5-HTRs (blue) binds the engineered GloSensor luciferase, switching the sensor to a more active conformation resulting in enhanced luminescence output. ( C ) Kinetics of signal following the addition of 5-HT (1 µM) to HEK293 cells co-transfected with luminescent cAMP sensor and individual schistosome 5-HT receptors. Open circles, cells not transfected with 5-HT receptor, colored circles represent measurements in cells transfected with Sm .5HTR L (black), ( Sh. 5HTR (blue) and Sj .5HTR (red). ( D ) Serotonin dose-response curves for each of the three receptors (colored circles) and HEK293 cells expressing the cAMP sensor alone (open circles). Data reflect mean ± standard error of at least 3 biological replicates.

    Journal: eLife

    Article Title: Coalescing beneficial host and deleterious antiparasitic actions as an antischistosomal strategy

    doi: 10.7554/eLife.35755

    Figure Lengend Snippet: ( A ) Confocal images showing cell surface expression in mammalian HEK293 cells of 5-HT receptors cloned from S. mansoni ( Sm .5HTR L ), S. haematobium ( Sh. 5HTR) and S. japonicum ( Sj .5HTR) localized by COOH-terminally tagged eGFP. Scalebar, 50 µm. ( B ) Schematic of luminescent cAMP sensor bioassay. cAMP generated by schistosome 5-HTRs (blue) binds the engineered GloSensor luciferase, switching the sensor to a more active conformation resulting in enhanced luminescence output. ( C ) Kinetics of signal following the addition of 5-HT (1 µM) to HEK293 cells co-transfected with luminescent cAMP sensor and individual schistosome 5-HT receptors. Open circles, cells not transfected with 5-HT receptor, colored circles represent measurements in cells transfected with Sm .5HTR L (black), ( Sh. 5HTR (blue) and Sj .5HTR (red). ( D ) Serotonin dose-response curves for each of the three receptors (colored circles) and HEK293 cells expressing the cAMP sensor alone (open circles). Data reflect mean ± standard error of at least 3 biological replicates.

    Article Snippet: To measure signaling output, GPCR activity was assessed using a luciferase based cAMP reporter (GloSensor 22F, Promega) engineered to undergo a conformational change on cAMP binding that increases luciferase activity ( ).

    Techniques: Expressing, Clone Assay, Bioassay, Generated, Luciferase, Transfection